I built an interactive analysis that separates cell-state composition from cell-intrinsic gene regulation in 1,714 acute-leukemia RNA-seq samples. Using an integrated 35-state bone marrow and thymus reference, we mapped AML, B-ALL, and T-ALL onto normal development and validated the results with matched single-cell data. We found that fusion-defined leukemias are often explained by differentiation state, while several mutations retain strong expression signals beyond composition. This helps distinguish where leukemic cells sit in development from what they express abnormally.
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