Frontier gene writers

Frontier gene writers is an agentic pipeline that discovers programmable recombinases and transposases for therapeutic gene insertion. Precise insertion of large therapeutic DNA payloads remains a bottleneck for genetic medicines. The pipeline queries MGnify Proteins for serine-recombinase domains (Pfam PF00239 + PF07508 core, PF13408 bonus), intersects the two searches (34 candidates), ranks 20 by filter score plus a bounded tie-break, and uses an LLM review to judge the top candidates. In the demo run, the top two candidates tied at 4.850 (effectively co-leaders, near-duplicate homologs), and the smallest top candidate (509 aa) was judged most delivery-friendly. The work is 100% dry-lab: code only, no wet-lab validation.