Talaria

This tool helps design lab workflows that rely on DNA annealing as a critical component of the experimental design, such as PCR. DNA annealing is calculated using a nearest-neighbor model and the delta H and delta S values to determine the percent-bound rates of oligonucleotides. Existing tools focus on calculating melting temperature (50% bound), even though they do all of the hard work of calculating the values needed to get percent bound, they don't expose it in the APIs. These tools also have licensing, which means that we can't just ask Claude to look at it and rewrite them. So I provided Claude links to some of the foundational papers in this field and asked it to build a new, more sophisticated version of these tools that better allows for lab scientists to design experiments that account for the entropy of the system instead of hiding it.