# Splice with Claude

- **Event:** [Built with Claude: Life Sciences](https://cerebralvalley.ai/e/built-with-claude-life-sciences)
- **When:** Jul 7 at 12:00 PM – Jul 14 at 12:00 AM (EDT)
- **Where:** Online
- **Team:** [SAFIYA KHURSHID](https://cerebralvalley.ai/u/soprich2)
- **GitHub:** https://github.com/mohsinahad/liver-cancer-splicing-survival
- **Demo video:** https://youtu.be/b9uC9Rmsp2s
- **Gallery:** https://cerebralvalley.ai/e/built-with-claude-life-sciences/hackathon/gallery
- **Page:** https://cerebralvalley.ai/e/built-with-claude-life-sciences/hackathon/gallery/276

I asked which RNA-splicing changes in liver cancer actually affect patient survival — and whether isoform switching correlated with survival rather than gene expression. Using only public data, I screened 23,909 splicing events in TCGA-LIHC against overall survival (382 significant, FDR<0.05), correlated splicing with matched protein abundance, and validated 18 survival-associated genes at the protein level in an independent cohort (CHCC-HBV deep proteome). I independently re-quantified tumor-vs-normal splicing with two orthogonal pipelines (SUPPA2 and LeafCutter), which agree on 90 genes (p=1.9×10⁻¹³). Running survival on the exact differentially-spliced events showed that 8 of 18 survival-defining genes, including the lead gene FN1, are prognostic through isoform switch alone, with no expression change: biology a conventional differential-expression analysis would miss. FN1's survival-linked exon is the IIICS/V oncofetal-fibronectin cassette, a splice-switching-ASO target hypothesis.

Why it matters: I'm a wet-lab RNA-splicing PI. This is a multi-cohort, dual-pipeline proteogenomic + survival study that would normally need a bioinformatician, a splicing-methods specialist, and a biostatistician over months. With Claude Science I ran the whole thing myself, in days, including catching and correcting my own false leads and validating across two cohorts. The expertise was always mine; what changed is I no longer depend on a team to act on it and/ or move the field forward.

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