# PerturbGate

- **Event:** [Built with Claude: Life Sciences](https://cerebralvalley.ai/e/built-with-claude-life-sciences)
- **When:** Jul 7 at 12:00 PM – Jul 14 at 12:00 AM (EDT)
- **Where:** Online
- **Team:** [Margarita Soloshenko](https://cerebralvalley.ai/u/Margosolo)
- **GitHub:** https://github.com/MargoSolo/perturbgate-perturbseq
- **Demo video:** https://drive.google.com/file/d/1QVZjKWSkjHtPjq03FXxN32L6DgIl_A4c/view?usp=drivesdk
- **Gallery:** https://cerebralvalley.ai/e/built-with-claude-life-sciences/hackathon/gallery
- **Page:** https://cerebralvalley.ai/e/built-with-claude-life-sciences/hackathon/gallery/111

PerturbGate is an open-data, reproducible pipeline that turns a genome-scale primary human CD4 T-cell Perturb-seq screen into evidence-gated mechanism hypotheses — not another ranking of hits. Each of 924 knockdowns is scored not by "does the cell change?" but by "does it reverse a donor-paired juvenile-arthritis disease direction?", then put through gates for technical validity, disease direction, an external patient cohort, and translatability. The result is an honest triad: PAK2, a strong reproducible cellular hit, was rejected as non-directional; RIPK1 is an unsupported comparator; RICTOR reverses the inflamed T-cell programme (+0.161 internal, +0.165 in an independent JIA cohort, 6/6 leave-one-donor-out) but is retained only as a mechanism hypothesis with an explicit modality gap  - never a validated target. Every number resolves to a committed frozen table; negatives and corrected results are first-class outputs. It recomputes on a laptop in minutes.

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Markdown version of https://cerebralvalley.ai/e/built-with-claude-life-sciences/hackathon/gallery/111. Site index for agents: https://cerebralvalley.ai/llms.txt · full text: https://cerebralvalley.ai/llms-full.txt